TGase 2 (Transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase), G-ALPHA-h, GNAH, Protein-glutamine gamma-glutamyltransferase 2, TG(C), TG2, TGM2, TGase C, TGase H, TGC, Tissue transglutaminase, Transglutaminase-2, Transglutaminas

Supplier:

United States Biological

Cat no: T8260-02

TGase 2 (Transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase), G-ALPHA-h, GNAH, Protein-glutamine gamma-glutamyltransferase 2, TG(C), TG2, TGM2, TGase C, TGase H, TGC, Tissue transglutaminase, Transglutaminase-2, Transglutaminas

Transglutaminase II catalyzes calcium-dependent post-translational modification of proteins by formation of an isopeptide bond within or between polypeptide chains. It is also known as TGC, tTG, type II-, Gh, cytosolic-, liver-, endothelial-, erythrocyte-, cellular-transglutaminase. Different tissues and cell types express varying amounts of tissue transglutaminase with a markedly hugh expression in rheumatoid lesions. It is implicated in programmed cell death, signal transduction, drugresistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation amd Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-1ug/ml for 2 hours at RT.\nImmunoprecipitation (Native and denatured): 2ug/mg protein lysate. Use Protein G.\nImmunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHUVEC cells, Breast carcinoma. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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SPECIFICATIONS

Catalog Number

T8260-02

Size

500ul

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, GP

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See T8260-02X

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Birckbichler PJ, et. al. Hybridoma, 1985, 4:179

Additional Info

Recognizes guinea pig Transglutaminase II between 77-85kD. Species Crossreactivity: human

Read more on Supplier website

SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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Hosts

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Reactivities

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