Transforming growth factor betas (TGFbs) mediate many cell-cell interactions that occur during embryonic development. Three TGFbs have been identified in mammals. TGF-b1, TGF-b2 and TGF-b3 (M.W 12.5) are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112aa polypeptide that remains associated with the latent portion of the molecule. This antibody does not cross react with TGF-b1 or TGF-b2.
Applications:
Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry: 1:100 for 10min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min.
Optimal dilutions to be determined by the researcher.
Positive Control:
Placenta
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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