Thymidylate Synthase (TYMS, TS, TSase, TMS, TYMS Protein, Tyms Thymidylate Synthetase, dTMP Synthase, EC 2.1.1.45, HsT422, MGC88736, OK/SW-cl.29)

The methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) is an essential step in the formation of thymine nucleotides (1,2, reviewed in 3). This process is catalyzed by thymidylate synthase (TS or TYMS), a homodimer composed of two 30kD subunits. TS is an intracellular enzyme that provides the sole de novo source of thymidylate, making it a required enzyme in DNA biosynthesis with activity highest in proliferating cells (1). Being the exclusive source of dTMP, TS is also an important target for anticancer agents such as 5-fluorouracil (5-FU) (1-5). 5-FU acts as a TS inhibitor and is active against solid tumors such as colon, breast, head, and neck. Studies have shown that patients with metastases expressing lower levels of TS have a higher response rate to treatment with 5-FU than patients with tumors that have increased levels of TS (5). TS expression may be useful in predicting overall patient survival, but the prognostic value of TS expression continues to be a subject of investigation for different types of cancers (6-10).\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunoprecipitation: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T5155-10K

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1.Johnston, P.G. et al. (1991) Cancer Res 51, 6668-76. 2.Aschele, C. et al. (2002) Ann Oncol 13, 1882-92. 3.Jackman, A.L. and Calvert, A.H. (1995) Ann Oncol 6, 871-81. 4.Van Triest, B. et al. (2000) J Histochem Cytochem 48, 755-60. 5.Johnston, P.G. et al. (1994) J Clin Oncol 12, 2640-7. 6.Kwon, H.C. et al. (2007) Ann Oncol 18, 504-9. 7.Allegra, C.J. et al. (2002) J Clin Oncol 20, 1735-43. 8.Allegra, C.J. et al. (2003) J Clin Oncol 21, 241-50. 9.Tsourouflis, G. et al. (2008) Dig Dis Sci 53, 1289-96. 10.Kim, S.H. et al. (2009) Am J Clin Oncol 32, 38-43.

Additional Info

Recognizes endogenous levels of total human Thymidylate Synthase. Species Crossreactivity: Hamster, monkey, mouse and rat.