TIE1 (TIE-1, Tyrosine Protein Kinase Receptor, Tunica Internal Endothelial Cell Kinase)

Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature). A family of receptor tyrosine kinases TIE1 and TIE 2 or Tek has been identified in vascular endothelium and hematopoietic cells. Mice lacking TIE 1 or TIE 2 are lethal. Ties may represent the earliest endothelial cell lineage marker and may regulate the endothelial cell proliferation, differentiation, and proper patterning during vasculogenesis. TIEs appear to be acting downstream of the VEGFRs. Tie-1 (human 1138 AA; mouse 1134 aa) is a type 1 membrane receptor protein specifically expressed in developing vascular endothelial cells. Tie-1 extracellular portion (25-279 aa) contains 3 fibronectin type III-like and 2 Ig-like C2-type, and 3-EGF-like domains. \n\nApplications:\nSuitable for use in Western Blot and ELISA. Other applications not tested. \n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml (ECL). NIH/3T3 cells can be used as positive control. TIE-1 ~135-140kD.\nELISA: 1:10,000-1:100,000. Using 50-100ng of control peptide/well.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T5498-65B

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, before the addition of glycerol to 40%.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Partenen J et al (1992) Mol. Cell. Biol. 12, 1698-1707; 2. Sato TN et al (1993) PNAS 90, 12056; I3. wama A et al (1993) BBRC 195, 301-309; 4. Korhonen J et al (1995) Blood 86, 1828-1835; 5. deVries C et al (1992) Science 255, 989-991.

Additional Info

Recognizes human TIE1. Species Sequence Homology: bovine, mouse - 100%.