TIMP-3 BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human TIMP-3 in cell culture supernates, serum, plasma (heparin, EDTA) and saliva.\n\nThe tissue inhibitors of metalloproteinases (TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy (SFD).\n\nAssay Range:\n156pg/ml-10,000pg/ml\n\nSensitivity:\n< 2 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP35625\n\nEntrez Gene ID:\n7078\n\nKit Components:\n96-well plate pre-coated with anti-TIMP-3, Human\nLyophilized recombinant TIMP-3, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

144059

Size

96Tests

References

1. Apte, S. S.; Mattei, M.-G.; Olsen, B. R.: Cloning of the cDNA encoding human tissue inhibitor of metalloproteinases-3 (TIMP-3) and mapping of the TIMP3 gene to chromosome 22. Genomics 19: 86-90, 1994.\n2. Stohr, H.; Roomp, K.; Felbor, U.; Weber, B. H. F.: Genomic organization of the human tissue inhibitor of metalloproteinases-3 (TIMP3). Genome Res. 5: 483-487, 1995.\n