Tissue cDNA Panel, Human Adult Normal, Reproductive 1, Testis, Epididymis, Penis, Seminal vesicle, Placenta, BioGenomics(TM)

BioSelect(TM) Tissue cDNA: cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection.\n\nThe cDNA panel is comprised of 5 tubes each containing 10ul of PCR Ready First Strand cDNAs from 5 tissues. These cDNAs are ideal for analyzing gene expression patterns and discovering novel genes. The cDNAs included in the panel are subject to source availability. All cDNAs in a panel are made under optimal and consistent conditions, which ensure that representation is maintained from total RNA to cDNA.\n\nThe First-Strand cDNA is synthesized from RNA isolated from a wide variety of documented human adult and fetel normal tissues, human diseased and tumor tissues, as well as from mouse, rat, monkey and plant tissues. Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 11ug total RNA was primed by an oligo dT primer and reverse transcribed by MMLV reverse transcriptase in 40ul final volume. RT reaction was stopped by heating at 65 degrees C for 10 minutes. The cDNA is in 1X RT buffer (1X RT Buffer: 50 mM Tris Cl, pH 8.3, 75mM KCl, 3mM MgCl2, 10mM DTT). \n\n1ul cDNA is sufficient for one PCR reaction. The 5' end of human clathrin cDNA (a 6kb gene) has been amplified by PCR from all of the cDNAs. \n\nFeatures:\nReady to use for PCR quantification of gene expression \nOligo dT primer used to ensure the entire 3' end of cDNA is present \nWith some cDNA used as templates, 12kb PCR amplicon was obtained to ensure intact cDNAs \nThe largest selection of cDNAs from different tissues on the market \nPlacenta cDNA is included in every panel as an interpanel control \nDocumentation of tissues' clinical histories available (additional cost) \nEconomical way to get cDNA from multiple tissues \n\nApplications:\nImmediate PCR amplification of known genes for their expression \nQuantification of low copy gene expression in multiple tissues \nIdentification of tissue-specific expression of target genes \nAnalysis of gene expression in rare tissues \nVerification of genetic mutation \nComparison of a specific gene between different tissues \nAnalysis of mRNA alternative splicing \n\nStorage Conditions:\nStore cDNA panel at -20C

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