Tissue Factor Pathway Inhibitor (TFPI, TFI, TFPI 1, TFPI1, Anti Convertin, Anti-convertin, Extrinsic Pathway Inhibitor, EPI, Lipoprotein-associated Coagulation Inhibitor, LACI)

This gene encodes a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nPeptide ELISA Titer: 1:64,000\nWestern Blot: 0.3-1ug/ml ~50kD band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 35.0kD according to NP_006278.1). In transfected HEK293 transiently expressing TFPI bands of approx. 38kD to 45kD are observed. These bands are not observed in the non-transfected HEK293. \nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T5576-07B

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by peptide affinity chromatography.

Isotype

IgG

References

1.Wun TC, Kretzmer KK, Girard TJ, Miletich JP, Broze GJ Jr.Cloning and characterization of a cDNA coding for the lipoprotein-associated coagulation inhibitor shows that it consists of three tandem Kunitz-type inhibitory domains. J Biol Chem 1988 May 5;263(13):6001-4

Additional Info

Recognizes human TFPI. This antibody is expected to recognize isoform a (NP_006278.1) only.

Alternative Names

tissue factor pathway inhibitor, lipoprotein-associated coagulation inhibitor, extrinsic pathway inhibitor, anti-convertin, OTTHUMP00000205431, OTTHUMP00000163472, TFPI1, TFI, LACI, EPI, TFPI, tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)