Tissue-type plasminogen activator (tPA) circulating in plasma exists in free forms as well as in tPA-inhibitor complexes. The underlying biochemical mechanism governing coagulation and fibrinolysis involves a controlled cascade of proteolytic reactions characterized by the conversion of serine protease zymogens to enzymes. Depending upon the initiating stimulus, the physiological outcome of these reactions is blood clot formation/dissolution (1-4). The relative concentrations of serine protease zymogen, enzyme, or enzyme-inhibitor complex in circulation will vary in accord with the state of vascular damage. Many conventional antigen-based clotting factor-specific immunoassays do not distinguish between zymogen and enzyme.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: Non-reducing conditions
Optimal dilutions to be determined by the researcher.
Recommended Pair for Sandwich ELISA:
Capture: T5600-10F Tissue Plasminogen Activator (tPA)
Detection: T5600-10G: Tissue Plasminogen Activator (tPA)
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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