TLR2 (Toll Like Receptor 2, CD282) (FITC)

Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. TLR2 has been identified as a receptor that \nis central to the innate immune response to lipoproteins of Gram-negative bacteria, several whole Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products. A functional interaction between TLR2 and TLR6 in the cellular response to various bacterial products has been discovered. The currently accepted paradigm regards TLR2 as an essential receptor for many eubacterial cell wall components, including lipoproteins and peptidoglycan. Bacterial species as diverse as mycobacteria, spirochetes, mycoplasma, Staphylococcus aureus, and Streptococcus pneumoniae have all been shown to mediate cellular activation via TLR2 (CD282). TL2.1 is a TLR2 function blocking antibody that is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human peripheral blood mononuclear cells.\n\nApplications:\nSuitable for use in Immunoassays, Western Blot, Immunohistochemistry, Immunoprecipitation, and Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:50\nFlow Cytometry: 1:50\nImmunohistochesmistry (frozen and paraffin): 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nFor long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

T8050-09V

Size

100ug

Applications

FC, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, NHP

Form

Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Lien, E et al; Toll-like receptor 2 functions as a pattern recognition receptor for diverse bacterial products. J Biol Chem 1999, 274: 33419 2. Flo, TH et al; Differential expression of Toll-like receptor 2 in human cells. J Leukoc Biol 2001, 69: 474 3. Lien, E et al; A novel synthetic acyclic lipid A-like agonist activates cells via the lipopolysaccharide/toll-like receptor 4 signaling pathway. Biol Chem 2001, 276: 1873 4. Flo, TH et al; Human toll-like receptor 2 mediates monocyte activation by Listeria monocytogenes, but not by group B streptococci or lipopolysaccharide. J Immunol 2000, 164: 2064 5. Faure, E et al; Bacterial lipopolysaccharide and IFN-gamma induce Toll-like receptor 2 and Toll-like receptor 4 expression in human endothelial cells: role of NF-kappa B activation. J Immunol 2001, 166: 2018 6. Wang, JE et al; Involvement of CD14 and toll-like receptors in activation of human monocytes by Aspergillus \nfumigatus hyphae. Infect Immun 2001, 69: 2402 7. Droemann, D et al; Toll-like receptor 2 is expressed by alveolar epithelial cells type II and macrophages in the human lung. Histochem Cell Biol 2003, 119: 103

Additional Info

Recognizes human Toll-like receptor 2 (TLR2, CD282). Species crossreactivity: cynomolgus monkey, rhesus monkey, marmosef monkey.

Alternative Names

CD282, Toll-like receptor 2