TLR4 (Toll-like Receptor 4, hToll, CD284)

Cat no: T8050-34J3

Supplier: United States Biological

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Additional Information

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The TLR4 cDNA codes for a protein consisting of 799aa with approximate molecular weight of 88kD (6). Applications: Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-2ug/10e6 cells Western Blot: 1-3ug/ml Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	T8050-34J3
Reactivities:	Human, Mouse, Rat
Hosts:	Mouse
Applications:	Flow Cytometry, Immunohistochemistry, Western Blot
Size:	100ug
Form:	Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
P type:	Mab
Isotype:	IgG2b,k
Purity:	Purified by Protein G affinity chromatography.
References:	1. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. Exp. Med. 187: 2097-2101 (1998). 2. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997). 3. Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta 1518 (1-2): 157-161 (2001). 4. Takeuchi O., Hoshino K, Kawai T, Sanjo H, Takada H, Ogawa K, Takeda K and Akira S. Immunity 11: 443 (1999). 5. Poltorak A, Riccardi-Castagnoli P, Citterio S, and Butler B. Proc. Natl. Acad. Sci USA 97: 2163-2167 (2000). 6. Medzhitov R, Preston-Hurlburt P and Janeway CA Jr. Nature 388 (6640), 394-397 (1997).
Additional info:	Recognizes human TLR4. Species Crossreactivity: mouse and rat. Species sequence homology: bovine, feline, chimpanzee, equine, porcine, sheep.

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TLR4 (Toll-like Receptor 4, hToll, CD284)

Cat no: T8050-34J3

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