TLR9 (Toll-like Receptor 9, CD289, UNQ5798/PRO19605) (Azide Free)

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). By using a BLAST search, Hemmi et al., 2000 (5) have identified and subsequently isolated a cDNA coding for TLR9. Gene knockout experiments suggest that TLR9 acts as a receptor for unmethylated CpG dinucleotides in the bacterial DNA (4). Human and mouse TLR9 share an overall aa identity of 75.5%. TLR9 is highly expressed in spleen.\n\nApplications:\nSuitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry (Intracellular): 2-5ug/1x10e6 cells\nWestern Blot: 1-3ug/ml\nImmunohistochemistry (frozen): 10-20ug/ml\nImmunohistochemistry: Paraffin\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman PBMC

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SPECIFICATIONS

Catalog Number

T8050-90A

Size

100ug

Applications

FC, ICC, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can, NHP

Form

Supplied as a liquid in PBS. Preservative free.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. exp. Med. 187: 2097-2101 (1998). 2. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997). 3. Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta 1518 (1-2):157-161 (2001) 4. Takeuchi O, Kawai T, Sanjo H, Copeland NG, Gilbert DJ, Jenkins NA, Takeda K, and Akira S. Gene 231: 59-65 (1999). 5. Hemmi H, Takeuchi O, Kawai T, Kaisho T, Sato S, Sanjo H, Matsumoto M, Hoshino K, Wagner H, Takeda K, and Shizo A. Nature 408: 740-745 (2000).

Additional Info

Recognizes human TLR9. Species Crossreactivity: canine, mouse, rat, rhesus monkey.