TLR9 (Toll-like Receptor 9, CD289, UNQ5798/PRO19605)

TLR9 is a 145-150kD member of the Toll-like receptor family of molecules. It is expressed by colonic epithelium, CD123+ plasmacytoid dendritic cells, and transitional B cells, and responds to unmethylated DNA CpG motifs that exhibit either a GTCGTT sequence (in human), or a GACGTT sequence (in mouse). TLR9 is found in the ER, and translocates to either the cell membrane, or to lysosomes where it binds bacterial DNA. Precursor human TLR9 is a type I transmembrane protein 1032aa in length. It possesses a 793aa extracellular region that contains 26 LRRs (aa26-818), plus a 193aa cytoplasmic domain. The full-length 150kD form is suggested to be ligand-binding but nonsignaling. The active form is believed to be an 80kD cleavage product found in the endosome compartment. There are multiple splice forms. One contains a deletion of aa2-16, a second possesses an alternate start site at aa58, while a third and fourth show alternative start sites aa23 and 24 upstream of the standard site. Over aa64-189, human TLR9 shares 76aa identity with mouse TLR9.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:3000\nImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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