TMB Substrate Reagent Set

The TMB Substrate Reagent Set contains 100 ml Substrate Reagent A and 100 ml Substrate Reagent B, sufficient for twenty 96-well plates. It is formulated with 3, 3', 5, 5' tetramethyl benzidine (TMB). The substrate can be catalyzed with peroxidase to produce a pale blue color which can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2N H2SO4 or 1M H3PO4 (resulting in a yellow color), and read at 450 nm.

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SPECIFICATIONS

Catalog Number

421101

Size

100 ml

Applications

ELISA

Dilutions

Substrate Reagent A should be clear and colorless. Substrate Reagent B should be colorless to a very light amber.TMB Substrate is the mixture of equal volumes of TMB Substrate Reagent A with TMB Substrate Reagent B. For example, for one plate, mix 6 ml Substrate Reagent A with 6 ml of Substrate Reagent B in a clean container. Mix immediately before use. After mixing the Reagents together, TMB substrate working solution should be colorless or very faint blue.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

The TMB Reagent Set (Substrate Reagent A and Substrate Reagent B) should be stored at 4C. Avoid prolonged exposure to light, contact with metal, air, or extreme temperatures.

Alternative Names

TMB Substrate

Regulatory Status

RUO