TNFA (Tumor Necrosis Factor, Cachectin, TNF-alpha, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF-a, TNF, TNFSF2) (FITC)

TNF-alpha is a homotrimeric 17kD protein, that interacts with either one of the two types of TNF-receptors, termed I and II, leading to receptor cross-linking and signal transduction. The receptors differ strongly in their intra-cellular signaling pathways. TNF-alpha was originally described as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Furthermore, TNF-alpha is found to be a central mediator in many inflammatory and immunological processes. It can be induced by various products of micro-organisms and by various cytokines leading to expression of a wide variety of cytokines. The pro-inflammatory properties of TNF-alpha play a central role in several auto-immune diseases such as rheumatoid arthritis and inhibition by neutralizing molecules have been shown to be beneficial in patients.\n\nApplications:\nSuitable for use in Immunohistochemistry, Flow Cytometry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: stains soluble TNF bound to TNF receptors. Cells were fixed in PBA containing 0.2% formaldehyde\nWestern Blot: A reduced sample treatment and 15% SDS-Page was used. The band size observed is 17kD\nImmunohistochemistry (Paraffin): Tissue sections were fixed in 5% formalin embedded in paraffin and cut into 2um sections\nImmunohistochemistry (Frozen): 6-um tissue sections were fixed in acetone for 10min\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

T9160-03W

Size

100ug

Applications

FC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, GP, NHP

Form

Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Bradding, P et al; Interleukin-4, -5, and -6 and Tumor Necrosis Factor-alpha in normal and asthmatic airways: Evidence for the human mast cell as a source of these cytokines. Am J Respir Cell Mol Biol 1994, 10: 471. 2. Bradding, P et al: TNFalpha is localized to nasal mucosal mast cells and is released in acute allergic rhinitis. Clin Exp Allergy 1995, 25: 406. 3. Gerspach, J et al; Detection of membrane-bound Tumor Necrosis Factor (TNF): an analysis of TNF-specific reagents. Microsc Res Tech 2000, 50: 243. 4. Laan van der, N et al; Tumor necrosis factor alpha (TNFalpha) in human skin: a comparison of different antibodies for immunohistochemistry. Arch Dermatol Res 2001, 293: 226. 5. Lee, E et al; Clinical and immunohistochemical characteristics of mucoceles. Ann Dermatol 2009, 21:345.

Additional Info

Recognizes human TNF-alpha. Species Crossreactivity: mouse, guinea pig and rhesus monkey.