The contraction of striated muscle is initiated by the binding of intracellular calcium to the troponin complex located in the thin filaments. The complex consists of the 3 different troponins; troponin C, troponin T, and troponin I. Troponin T is the tropomyosin binding subunit of troponin which confers calcium sensitivity to striated muscle actomyosin ATPase activity. TNNT3 (Troponin T type 3 (skeletal, fast)) is one of 3 identified troponin T proteins which are encoded by different genes.
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.