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Total Phosphatidic Acid Fluorometric Assay Kit

Cat no: 700240

Total Phosphatidic Acid Fluorometric Assay Kit

PA is a common phospholipid that is a major constituent of cell membranes and a central intermediate for the synthesis of membrane lipids and storage lipids. Besides de novo synthesis. Cayman's Phosphatidic Acid Assay provides a fluorescence-based method for measuring PA. Lipase is used to hydrolyze PA in cellular lipids to glycerol-3-phosphate, which is then oxidized by glycerol-3-phosphate oxidase to generate H2O2. In the presence of peroxidase, H2O2 reacts with ADHP (10-acetyl-3,7-dihydroxyphenoxazine) to yield the highly fluorescent compound resorufin (excitation 530-540 nm and emission 585-595 nm).

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SPECIFICATIONS

Catalog Number

700240

Form

96 Well

P Type

Assay Kits|Small Molecule Detection (Non-EIA)|Phospholipids

Weight

0

Storage Temp

-20

Shipping Temp

-20

Additional Info

Phosphatidic acid (PA) is a common phospholipid that is a major constituent of cell membranes and a central intermediate for the synthesis of membrane lipids and storage lipids. Cayman's Phosphatidic Acid Fluorometric Assay provides a fluorescence-based method for measuring PA. Lipase is used to hydrolyze PA in cellular lipids to glycerol-3-phosphate. A coupled enzyme reaction system generates the highly fluorescent compound resorufin (excitation 530-540 nm and emission 585-595 nm).

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