Home  >  Products  >  Transcription Cofactor Vestigial-like Protein 2 (VGL2, Vgl-2, Protein VITO1, Vestigial Like 2 (Drosophila), VGLL2, VITO1)
Transcription Cofactor Vestigial-like Protein 2 (VGL2, Vgl-2, Protein VITO1, Vestigial Like 2 (Drosophila), VGLL2, VITO1)

Transcription Cofactor Vestigial-like Protein 2 (VGL2, Vgl-2, Protein VITO1, Vestigial Like 2 (Drosophila), VGLL2, VITO1)

Cat no: T8194-65

Supplier: United States Biological
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VGLL2 is the master regulator in the wing development in Drosophila, which acts through a complex by interacting with scalloped, a homolog of vertebrate TEF proteins. Northern and dot blot analyses of human adult tissues detected VGLL2 expression in skeletal muscle. In situ hybridization revealed VGLL2 expression in differentiating somites and branchial arches during mouse embryogenesis. VITO1 is the clone of VGLL2. It contains a scalloped interaction domain (SID). VITO1 is more highly expressed in differentiated myotubes from the mouse C2C12 myogenic cell line than in undifferentiated cells. During differentiation in mouse C2C12 cell line, VGLL2 mRNA levels increase and VGLL2 protein translocates from the cytoplasm to the nucleus. Interference with VITO1 expression by small interfering RNA attenuated differentiation in C2C12 muscle cells. Therefore, this protein is an important component of the myogenic program. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Skeletal muscle Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: T8194-65
Reactivities: Human, Mouse, Bovine, Feline, Porcine
Hosts: Rabbit
Applications: Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by Protein A affinity chromatography.
References: 1. Mielcarek, M. et al. Mech. Dev. 119S:S269-S274 (2002). 2. Maeda, T. et al. J. Biol. Chem. 277:48889-48898 (2002). 3. Gunther, S. et al. Nucleic Acids Res. 32:791-802 (2004).
Additional info: Recognizes human VGLL2/VITO1. Species Crossreactivity: feline, chimpanzee, bovine, equine, porcine, rhesus monkey. Species sequence homology: mouse, xenopus, chicken, opossum.

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