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Transcription Factor E3 (TFE3, Class E Basic Helix-loop-helix Protein 33, bHLHe33)

Transcription Factor E3 (TFE3, Class E Basic Helix-loop-helix Protein 33, bHLHe33)

Cat no: T8195-99F

Supplier: United States Biological
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The DNA-binding factor TFE-3 contains adjacent helix-loop-helix (HLH) and leucine zipper (LZ) domains flanked by an upstream basic region. TFE-3 is ubiquitously expressed and can directly associates with DNA as either homodimers or heterodimers formed with two related proteins, TFEB or TFEC. TFE-3 binds and activates the microE3 motif of the immunoglobulin heavy-chain enhancer to induce B- cell-specific gene transcription and DNA recombination. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: T8195-99F
Reactivities: Human, Mouse, Bovine, Canine
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Henthorn PS, Stewart CC, Kadesch T, Puck JM. The gene encoding human TFE3, a transcription factor that binds the immunoglobulin heavy-chain enhancer, maps to Xp11.22. Genomics. 1991 Oct;11(2):374-8
Additional info: Recognizes human TFE3. Species sequence homology: Bovine, canine and mouse.

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