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Transcription Factor EB (TCFEB, TFEB, Alpha TFEB, Alpha-TFEB, bHLHe35, T Cell Transcription Factor EB)

Transcription Factor EB (TCFEB, TFEB, Alpha TFEB, Alpha-TFEB, bHLHe35, T Cell Transcription Factor EB)

Cat no: T8196-01D

Supplier: United States Biological
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Transcription factors of the basic helix-loop-helix zipper (bHLH-ZIP) family contain a basic domain, used for DNA binding, and HLH and ZIP domains, both used for oligomerization. TFEB was isolated from a human B-cell cDNA library using a binding sequence from the adenovirus major late promoter. This transcription factor probably binds to the USF/MLTF site and probably recognizes E-box sequences in the heavy-chain immunoglobulin enhancer. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Positive Control: NIH-3T3 lysate Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: T8196-01D
Reactivities: Human, Mouse
Hosts: Goat
Applications: Western Blot
Size: 100ug
Form: Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: Steingrimsson E et al. Murine chromosomal location of five bHLH-Zip transcription factor genes. Genomics 28:179-83 (1995).
Additional info: Species Crossreactivity: Crossreacts with Mouse. Expected to crossreact with Human due to sequence homology.

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