Transcription Factor EB (TFEB, Transcription Factor EB, TCFEB, T-cell Transcription Factor EB)

Alternate Name: TFEB, transcription factor EB, TCFEB, T-cell transcription factor EB\n\nDomain Information: Helix-loop-helix DNA-binding domain\n\nGene Ontology Terms: transcription factor activity; regulation of transcription, DNA-dependent; nucleus\n\nApplication(s): Suitable for use in ELISA, Western Blot. Other applications not tested.\nWestern blot: Approx 50-55kD band seen in NIH-3T3 lysate. Recommended for use at 0.5-2ug/ml.\n\nStaining (1ug/ml) of NIH-3T3 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPeptide Blocking:\nCorresponding peptide is available for Peptide Blocking studies. See T8196-50A-P. Antibody

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SPECIFICATIONS

Catalog Number

T8196-50A

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

Steingrimsson E, Sawadogo M, Gilbert DJ, Zervos AS, Brent R, Blanar MA, Fisher DE, Copeland NG, Jenkins NA. , Murine chromosomal location of five bHLH-Zip transcription factor genes., Genomics. 1995 Jul 20;28(2):179-83.

Additional Info

Recognizes Human, Mouse TFEB