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Transforming Growth Factor beta (TGFb)

Cat no: T8250-12E

Transforming Growth Factor beta (TGFb)

Applications: \nSuitable for use in ELISA, Western Blot and Neutralization. Other applications not tested.\n\nRecommended Dilution:\nNeutralization: Neutralizes TGF-b activity in vitro inhibition assay of CCL/64 cell growth and also neutralize the growth promoting action of TGF-b in the NRK-49F colony forming assays. The effect of microinjection of this MAb into one blastomere of two cell stage Xenopus embryos indicated that this antibody was able to neutralize effectively the bioactivity of TGF-b in vivo. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8250-12E

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as liquid in PBS, pH 7.0, 40% glycerol..

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

Additional Info

Western Blot demonstrated reactivity to either dimeric (25kD) or monomeric (12.5kD) molecules of TGF-b under non-reducing and reducing condition, respectively. Recognizes both human platelet-derived and recombinant TGF-b in enzyme-linked in ELISA.

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