Trk B, phosphorylated (Tyr817) (Tyrosine Kinase Receptor B)

There is increasing evidence that neutrophins and their receptors play an important role in regulating development of both the central and the peripheral nervous systems (1). The binding of neurotrophin ligands to their respective Trk cellular receptors initiates intracellular signals essential for the growth and survival of neurons. The site of neurotrophin binding has been located to the fifth extracellular domain of the Trk receptor, with this region regulating both the affinity and specificity of Trk receptor:neurotrophin interaction (2). The TrkB receptor tyrosine kinase and its ligand, BDNF, have an essential role in certain forms of synaptic plasticity. Results provide evidence that TrkB mediates hippocampal plasticity via recruitment of PLCgamma, and by subsequent phosphorylation of CaMKIV (3). Also, TrkB has been found to be essential to the development of GABAergic neurons and regulates synapse formation in addition to its role in the development of axon terminals (4).\n\nApplications: \nSuitable for use in Western Blotting, Immunoprecipitation, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:5000 -1:10,000\nImmunocytochemistry: 1:50\nImmunoprecipitation: 1:40\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

T8663-22B

Size

100ul

Applications

ICC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1.Nakagawara A, et al. Genomics 25(2):538-46, 19952.Banfield MJ, et al. Structure 9(12):1191-9, 20013.Minichiello L, et al. Neuron 36(1):121-37, 20024.Rico B, et al. Nat Neurosci 5(3):225-33, 2002

Additional Info

Recognizes human TrkB phosphorylated at Y817. Species crossreactivity: rat.

Alternative Names

Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, GP145-TrkB, Trk-B