Troponin 1

Troponin I, one of 3 subunits that form the troponin complex of the thin filaments of striated muscle, is the inhibitory component of troponin, the thin filament regulatory complex in striated muscle. Separate genes encode cardiac-specific fast and slow skeletal-specific isoforms of this protein (1). Unlike other contractile proteins, the cardiac isoform of troponin I (TnIc) is expressed only in cardiac muscle and therefore offers a model for cardiac-specific expression. It is also subject to developmental regulation with increased expression occurring at the time of birth (2). TnIc, a sensitive and specific marker of myocardial cell injury, is useful in diagnosing and assessing prognosis in acute coronary syndromes. Studies report that TnIc is elevated in severe heart failure and may predict adverse outcomes (3).\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1,000,000\nImmunohistochemistry: 1:250- 1:500\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

T8665-15D

Size

100ul

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Hunkeler NM, et al. Circ Res. 69(5):1409-14, 1991. 2. Bhavsar PK, et al. Genomics 35(1):11-23, 1996. 3. Horwich TB, et al. Circulation 108(7):833-8, 2003.

Additional Info

Recognizes human Troponin 1.

Alternative Names

Cardiac troponin I