Troponin I, Cardiac, Human (cTnI, TNNC1) BioAssay(TM) ELISA Kit

Troponin complex consists of three components troponin I, T and C. The complex along with tropomyosin is located on the actin filament and is essential for the calcium-mediated regulation of skeletal and cardiac muscle contraction. Three isoforms of troponin I have been described for striated muscle. Two isoforms are characteristic for fast and slow skeletal fibers and one isoform for cardiac muscle. Troponin I consists of 181-211aa residues, and the cardiac isoform is larger due to the presence of an additional N-terminal peptide which has a biologically important function in the interactions of troponin I and troponin C. The main function of troponin I is the inhibition of actomyosin ATPase activity. Cardiac troponin I and T are sensitive biomarkers of myocardial injury and have become central to the diagnosis of myocardial infarction. They are elevated in many clinical syndromes associated with direct myocardial injury, myocardial ischemia, or ventricular strain. They are also released in a number of clinical situations in which thrombotic complications of coronary artery disease. These situations include conditions like pulmonary embolism, sepsis, myocarditis, and acute stroke.\n\nIntended Use:\nHuman Troponin I ELISA kit is to be used for the in vitro quantitative determination of human Troponin I in human serum, human plasma, buffered solution, or cell lysate. This kit has been configured for research use only and is not to be used in diagnostic procedures.\n\nSensitivity:\nThe minimal detectable dose of human Troponin I was calculated to be 0.38ng/ml, by subtracting two standard deviations from the mean of 10 zero standard replicates (ELISA buffer, S0) and intersecting this value with the standard curve obtained in the same calculation.\n\nSpecificity:\nThe following substances were tested and found to have no cross-reactivity: cardiac Troponin T and cardiac Troponin C. Muscle Troponin I had slight cross-reactivity.\n\nTest Principle:\nThe design of this assay is based on a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to human Troponin I. Samples are pippetted into these wells. Nonbound Troponin I and other components of the sample should be removed by washing, then biotin- conjugated monoclonal antibody specific to Troponin I added. In order to quantitatively determine the amount of Troponin I present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) should be added to each microplate well. The final step, a TMB-substrate solution added to each well. Finally, a sulfuric acid solution is added and the resulting yellow colored product is measured at 450nm. Since the increases in absorbency is directly proportional to the amount of captured Troponin I.\n\nKit Components:\nMicroplate: 1x96 wells\nIncubation buffer: 1x30ml\nWashing buffer, (10x): 1x100ml\nStandard protein: 1 glass vial lyophilized\nStandard/sample dilution buffer: 1x25ml\nSecond antibody: 1 glass vial lyophilized\nAV-HRP, (100x): 1x150ul\nSecondary antibody/AV-HRP dilution buffer: 1x25ml\nSubstrate (TMB): 1x20ml\nStop solution: 1x20ml\n\nStorage and Stability:\nStore components at 4 degrees C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T8665-15P

Size

96Tests

References

1. From RE Jr et al. Cardiac troponins in the intensive care unit: common causes of increased levels and interpretation. 2007, Crit Care Med. 35(2):584-588. 2. Babuin L and Jaffe AS. Troponin: the biomarker of choice for the detection of cardiac injury. 2005, CMAJ. 173(10):1191-1202. 3. Hunkeler NM et al. Troponin I isoform expression in human heart. 1991, Circ. Res. 69;1409- 1414.