TSC2 (Tuberous Sclerosis Complex 2, Tuberous Sclerosis 2 Protein, FLJ43106, LAM, TSC4, Tuberin)

Tuberous sclerosis complex (TSC) is an autosomal dominant genetic disorder, which is characterized by seizures, mental retardation and hamartomatous in multiple organs. The genetic disorder is caused by the mutation in two genes - TSC1 and TSC2. The TSC2 gene is located on the chromosome 16p13.3, and it encodes the protein tuberin(1). Tuberin can interact with hamartin, encoded by TSC1 gene, to form a physical and functional complex(2). Tuberin, together with hamartin, are widely localized within epithelial cells, lymphocytes, endocrine glands and central nervous system (CNS) throughout the both normal body and TSC patients(1). Several independent investigations have demonstrated that tuberin is directly phosphorylated by Akt(2,3) and is further involved in stimulating cell growth, which provide an important link between tuberin and growth factor signaling. The loss of tuberin is associated with cell cycle deregulation and tumor suppression(4,5).\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 0.1-1ug/ul \nWestern Blot: 1-3ug/ml \nImmunoprecipitation (Native): 5-10ug/ml \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

T9100-30

Size

200ul

Applications

ELISA, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. Michael W, et al. Brain Pathology 9:445-454, 1999. 2. Inoki K, et al. Nat Cell Biol 4(9):648-657, 2002. 3. Manning BD, et al. Mol Cell 10(1):151-162, 2002. 4. Hunter DS, et al. Cancer Res 1;62(13):3766-3772, 2002. 5. Yoon HS, et al. Am J Physiol Renal Physiol 283(2):F262-270, 2002.

Additional Info

Recognizes human tuberin encoded on tuberous sclerosis-2 (TSC2) gene with ~200kD. It does not crossreact with hamartin encoded on tuberous sclerosis-1 (TSC1) gene. Reactivity is con?rmed with tuberin and hamartin overexpressed cell lysates.