Tubulin, beta (Beta Tubulin, Tubulin beta Polypeptide, Tubulin beta-2 Chain, DAQB-47P19.9-001, dJ40E16.7, M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB, TUBB Protein, TUBB 1, TUBB1, TUBB 2, TUBB2, TUBB 5, TUBB5, XXbac-BCX111D4.2-001)

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunocytochemistry: 3% paraformaldehyde-0.5% Triton X-100 and methanol-acetone. \nWestern Blot: 0.5-1ug/ml using whole extract of cultured rat adrenal pheochromocytoma (PC-12) cells.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

T9155-01D

Size

100ul

Applications

ELISA, ICC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, Rabbit

Form

Supplied as a liquid in 0.01M PBS, pH 7.4, 1% BSA, 15mM sodium azide.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG1

References

Audebert S et al. The carboxy-terminal sequence Asp427-Glu432 of beta-tubulin plays an important function in axonemal motility. Eur J Biochem 261:48-56 (1999). Gingras D et al. Molecular cloning and characterization of a radial spoke head protein of sea urchin sperm axonemes: involvement of the protein in the regulation of sperm motility. Mol Biol Cell 9:513-22 (1998). Roach MC et al. Preparation of a monoclonal antibody specific for the class I isotype of beta-tubulin: the beta isotypes of tubulin differ in their cellular distributions within human tissues. Cell Motil Cytoskeleton 39:273-85 (1998). Banerjee A et al. Increased microtubule assembly in bovine brain tubulin lacking the type III isotype of beta-tubulin. J Biol Chem 265:1794-9 (1990).

Additional Info

Recognizes all mouse brain beta tubulin isoforms (beta and beta subpopulations), and sea urchin beta 2 tubulin. Species Crossreactivity: human, rat, bovine, chicken, hamster, Chlamydomonas reinhardtii, canine, rabbit, dinoflagellate (Oxyrrhis marina) and Sea Urchin (Lytechinus pictus and Paracentrotus lividus).