Tumor Necrosis Factor Receptor Associated Factor 6, CT (TRAF-6)

TRAFs (Tumor necrosis factor receptor-associated factors) are cytoplasmic adaptor proteins that associate with tumor necrosis factor receptors (TNFR), thus mediating tumor necrosis factor (TNF) induced signaling. The TRAF family consists of six members (TRAF1-6).1-3 TRAF proteins play an important role in regulating cellular death and cellular response to stress by mediating stimulation of AP-1 family through activation of MAP kinases, including JNK/SAPKs, ERKs and p38s.4 They are also involved in the regulation of inflammatory and anti-apoptotic responses through activation of the transcription factors of NF-kB.5 TRAF6 possesses several binding motifs in CD40, TRANCE-R and IRAK suggesting a crucial role in TRANCE-R signaling. It is involved in the activation of the anti-apoptotic serine/threonine kinase Akt/PKB through TRANCE-R.6 Due to its specific receptor-binding TRAF-C domain, TRAF6 is the only member of the TRAF family that also participates in signal transduction of the interlukin-1 receptor (IL-1R)/Toll-like receptor (TLR) superfamily.1,7,8 TRAF6 plays a crucial role in adaptive immunity, innate immunity, bone homeostasis, perinatal and postnatal survival, and cytokine signaling.9\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blotting: 1-3ug/ml\nImmunoprecipitation: 7ug/reaction\nOptimal dilutions to be determined by the researcher.\n\nPositive Controls: \nHuman Hela, HEK293, Jurkat, and mouse NIH3T3 cell lysates.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

T9161-65

Size

50ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Cao Z, et al. Nature 383:443-446, 1996. 2. Nakano H, et al. J Biol Chem 271:14661-14664, 1996. 3. Rothe M, et al. Cell 78:681-692,1994. 4. Karin M. Philos Trans R Soc Lond B Biol Sci 351:127-134, 1996 5. Darnay BG, et al. J Biol Chem 274:7724-7731, 1999. 6. Wong BR, et al. Mol Cell 4:1041-1049, 1999. 7. Aderem A and Ulevitch RJ. Nature 406:782-787, 2000. 8. Chung JY, et al. J Cell Sci 115:679-688, 2002. 9. Ye H, et al. Nature 418:443-447, 2002.

Additional Info

Recognizes human TRAF6 proteins. On Western blots, it identifies a band at ~55kD and a band at ~60kD. The two bands may represent the two reported forms of this protein (522 and 530 amino acids). Species Crossreactivity: mouse.