Tumor Necrosis Factor Receptor Associated Factor 6 (TRAF-6)

Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g. RANK, CD30, CD40, etc.) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6) (1-4). Recently it has been shown that TRANCE/OPGL activates the antiapoptotic serine/threonine kinase Akt/PKB through a signaling complex involving c-Src and TRAF6 (5). Mice deficient in TNF receptor-associated factor 6 (TRAF6) are osteopetrotic with defects in bone remodeling and tooth eruption due to impaired osteoclast function (6). Like TRAF2 and TRAF3, TRAF6 is also essential for perinatal and postnatal survival. These findings establish unexpectedly diverse and critical roles for TRAF6 in perinatal and postnatal survival, bone metabolism, LPS, and cytokine signaling. RANK (TRANCE receptor) interacts with various TRAFs through distinct motifs and activates NF-kB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kB activation (7). Over-expression of TRAF6 activates JNK, p38, or IKK in the absence of extracellular stimulation. \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000\nJurkat or NIH3T3 cell lysate can be used as positive control and an approximately 54kD band is detected. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T9161-80

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Rothe M, et al. Cell 78: 681-692 (1994). 2. Cheng G, et al Science 267: 1494-1498 (1995). 3. Nakane H, et al . J. Biol. Chem. 271: 14661-14664 (1996). 4. Cao Z, Xiong J, Takeuchi M, and Kurama, T. Nature 383: 443-446 (1996). 5. Wong BR,et al . Mol. Cell 6:1041-1049 (1999). 6. Lomaga MA, Yeh WC, Sarosi I, Duncan GS, et al. Genes Dev 13(8): 1015-1024 (1999). 7. Darnay BG, et al J Biol Chem 274 (12): 7724-7731 (1999).

Additional Info

Recognizes human Tumor Necrosis Factor Receptor Associated Factor 6 (TRAF-6)