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Ubiquitin-conjugating Enzyme E2 D1, Recombinant, Human, His-tag (UBE2D1, Stimulator of Fe Transport, SFT, UBC4/5 Homolog, UBC5A, UbcH5, UBCH5A, Ubiquitin Carrier Protein D1, Ubiquitin-conjugating Enzyme E2(17)KB 1, Ubiquitin-conjugating Enzyme E2-17kD 1,

Cat no: U1000-86N1

Ubiquitin-conjugating Enzyme E2 D1, Recombinant, Human, His-tag (UBE2D1, Stimulator of Fe Transport, SFT, UBC4/5 Homolog, UBC5A, UbcH5, UBCH5A, Ubiquitin Carrier Protein D1, Ubiquitin-conjugating Enzyme E2(17)KB 1, Ubiquitin-conjugating Enzyme E2-17kD 1,

UbcH5a is highly homologous to the S. cerevisiae Ubc4/5 family of E2 enzymes demonstrated to be essential for degradation of regulatory and abnormal proteins. UbcH5a, together with UbcH5b and UbcH5c, are the most active class of E2 enzymes in cell extracts and are associated with regulation of a number of transcription factors. Specifically, UbcH5a stimulates the conjugation of ubiquitin to the tumor suppressor p532. Useful for in vitro ubiquitinylation reactions. Typical enzyme concentration to support conjugation in vitro is 100nM to 1uM depending upon conditions.\n\nSource:\nRecombinant corresponding to human UbcH5a with His-tag, expressed in E. coli.\n\nMolecular Weight:\n~17kD\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

U1000-86N1

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 0.5mM DTT.

Purity

~95% (SDS-PAGE)

Alternative Names

EC=6.3.2.19

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

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Applications

IF

Hosts

Mouse

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Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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