Ubiquitin

Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process. This process labels the target protein as a degradation marker for 26S lysosome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1. The activated ubiquitin is subsequently transfered to the ubiquitin-carrier protein E2. E2 is processed to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormal cases. Several examples (i.e. IkappaB, p53, cdc25a and Bcl-2) have been shown to be targets for the ubiquitination-proteosome process for regulation of cell cycle progression and differentiation, cell stress response and apoptosis (4-7).\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunohistochemistry (Paraffin): 1:750\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

U1000-07A

Size

100ul

Applications

IHC, WB

Hosts

Mouse

Reactivities

Bov

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, < 0.02% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. Ciechanover, A., (1998) EMBO J. 17:7151-7160. 2. Hochstrasser, M., (2000) Nat. Cell Biol. 2:E153-E157. 3. Hochstrasser, M., (2000) Science 289:563-564. 4. Bernardi, R., et al., (2000) Oncogene 19:2447-2454. 5. Aberle, H., et al., (1997) EMBO J. 16:3797-3804. 6. Salomoni, P., Pandolfi, P.P., (2002) Nat. Cell Biol. 4:E152-E153. 7. Jesenberger, V., Jentsch, S., (2002) Nat. Rev. Mol. Cell Biol. 3:112-121.

Additional Info

Recognizes ubiquitin, polyubiquitin and ubiquitinated proteins. Species Crossreactivity: All species