UEV1 (Mms2, Ubiquitin-conjugating enzyme variant 1)

In the S.cerevisiae yeast proteome, 13 different E2 ubiquitin-conjugating enzymes (Ubcs) exist, all sharing a conserved Cys residue that acts as the active site in a region known as the Ubc-motif. The yeast Mms2 protein belongs to a family of noncanonical E2 proteins, collectively called ubiquitin-conjugating enzyme variants (UEVs), that lack the defining active-site Cys of classical Ubc family members. Consistent with the absence of the active-site Cys, Mms2 does not appear to possess Ubc activity. However, the yeast Mms2 protein has been identified as a positive regulator of the assembly of poly-ubiquitin chains via the Lys63 residue of ubiquitin, which implicates it in the ubiquitin-proteasome protein degradation pathway. Yeast Mms2 also functions in the RAD6 pathway of error-free DNA postreplication repair (PRR);2 the formation of a heterodimer with the Ubc13 E2 partner is required for this activity. Two human homologs of the yeast Mms2 protein, hMms2 and CROC1, have been identified. CROC1 and hMms2 have\nbeen observed to transcriptionally activate the promoter of the c-fos protooncogene, which protects cells from DNA damage. Human Mms2 is also known to form a stable complex with human Ubc13, suggesting that the human protein may have an analogous role in error-free DNA repair to its yeast homolog. The human Mms2-Ubc13 complex plays a role in NFkB signal transduction.\n\nApplications:\nSuitable for use in Immunofluorescence, Western Blot and ELISA. Other applications have not been tested.\n\nRecommended Dilution:\nImmunofluorescence: 2-5ug/ml Mouse, human\nWestern Blot: 1-3ug/ml Human\nELISA: 0.1

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SPECIFICATIONS

Catalog Number

U1075-01

Size

100ug

Applications

ELISA, IF, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A chromatography

Isotype

IgG1,k

Additional Info

Specific for the ~21kD human Mms2 (UEV1) protein. Species Crossreactivity: mouse. Reactivity has been confirmed with human HepG2 cell lysates and mouse NIH 3T3 cells.