Urotensin II (UII)

Urotensins are neuropeptide secreted from the urophysis in the caudal neurosecretory system of the fishes. Two bioactive Urotensins, U1 and UII have been cloned from various species including mammals. UI is 41aa peptide, which showed prolonged hypotensive effects in mammals. It is structurally similar to mammalian corticotropin-releasing factor. UII is a vasoactive 'somatostatin-like" 12-aa cyclic peptide with similarity to somatostatin-14. Human UII is composed of only 11aa residues, while fish and frog UII possess 12 and 13aa, respectively. The cyclic region of the UII, which is responsible for the biological activity of the peptide, has been fully conserved from fish to human. UII expression has been observed predominantly in the spinal cord.\n\nRecently, an orphan human G-protein coupled receptor homologous to rat GPR14 has been identified as the receptor for UII. GPR14 is expressed predominantly in cardiovascular tissue. Human UII effectively stimulated total peripheral resistance in anesthetized non-human primates, a response associated with profound cardiac contractile dysfunction. GPR14 (rat 386aa and human 389aa, ~75% homology) contains 7 transmembrane domains. It is most closely related to somatostatin receptor4. GPR14 is mostly expressed in the heart and pancreas, while low expression was detected in the brain.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:3000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

U2800-01E

Size

20ul

Applications

ELISA

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute in ddH2O.

P Type

Pab

Purity

Serum

References

1. Heller J, Schepke M, Neef M, Woitas R, Rabe C, Sauerbruch T. Increased urotensin II plasma levels in patients with cirrhosis and portal hypertension. J Hepatol 2002;37:767-72. \n2. Dschietzig T, Bartsch C, Pregla R, Zurbrugg HR, Armbruster FP, Richter C et al. Plasma levels and cardiovascular gene expression of urotensin-II in human heart failure, Regul Pept 2002;110:33-8. \n3. Dschietzig T, Richter C, Bartsch C, Bohme C, Heinze D, Ott F et al. Flow induced pressure differentially regulates endothelin-1, urotensin II, adrenomedullin, and relaxin in pulmonary vascular endothelium. Biochem Biophys Res Commun 2001;289:245-51.

Additional Info

Recognizes synthetic human Urotensin II. There were no cross reactivities obtained with human Endothelin (aa 1-21) (< 0,1%); human big Endothelin (aa 1-38) (< 0,1%); human Urodilatin; human ANP (1-28) (< 0,1%); human BNP (aa 1-32) (< 0,1%); Adrenomedulin (aa 1-52) (< 0,1%); Relaxin (< 0,1%); and Somatostatin (< 0,1%).