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USP14, Recombinant, Human, aa1-494, His-Tag (TGT, Ubiquitin Thioesterase 14)

Cat no: 138771

USP14, Recombinant, Human, aa1-494, His-Tag (TGT, Ubiquitin Thioesterase 14)

USP14 is a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. \n\nSource:\nRecombinant protein corresponding to aa1-494 from human USP14 at N-terminal, fused to His-tag expressed in E.coli. \n\nMolecular Weight: \n~58.5kD (517aa)\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MGSMPLYSVT VKWGKEKFEG VELNTDEPPM VFKAQLFALT GVQPARQKVM VKGGTLKDDD WGNIKIKNGM TLLMMGSADA LPEEPSAKTV FVEDMTEEQL ASAMELPCGL TNLGNTCYMN ATVQCIRSVP ELKDALKRYA GALRASGEMA SAQYITAALR DLFDSMDKTS SSIPPIILLQ FLHMAFPQFA EKGEQGQYLQ QDANECWIQM MRVLQQKLEA IEDDSVKETD SSSASAATPS KKKSLIDQFF GVEFETTMKC TESEEEEVTK GKENQLQLSC FINQEVKYLF TGLKLRLQEE ITKQSPTLQR NALYIKSSKI SRLPAYLTIQ MVRFFYKEKE SVNAKVLKDV KFPLMLDMYE LCTPELQEKM VSFRSKFKDL EDKKVNQQPN TSDKKSSPQK EVKYEPFSFA DDIGSNNCGY YDLQAVLTHQ GRSSSSGHYV SWVKRKQDEW IKFDDDKVSI VTPEDILRLS GGGDWHIAYV LLYGPRRVEI MEEESEQ\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

138771

Size

100ug

Form

Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.2M sodium chloride, 1mM DTT, 20% glycerol.

Purity

~90% (SDS-PAGE)

References

1. Shinji S, Naito Z, et al. (2009). Oncol Rep. 15(3):539-43. 2. Mines MA, Goodwin JS, et al. (2009). J Biol Chem. 284(9):5742-52.

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