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UTF1 (Undifferentiated Embryonic Cell Transcription Factor 1)

Cat no: 030714

UTF1 (Undifferentiated Embryonic Cell Transcription Factor 1)

Undifferentiated transcription factor-1 (UTF-1) is used as marker for the undifferentiated state of pluripotent stem cells.UTF1 is a chromatin-associated protein with core histone-like properties. UTF1 further acts as a transcriptional repressor and is required for proper differentiation of pluripotent cells.\n\nUTF1 (undifferentiated embryonic cell transcription factor 1) is a 47-57kD transcriptional coactivator. It is expressed mainly in embryonic stem cells, is regulated by Oct-3/4, and promotes rapid cell proliferation. Human UTF1 is a 341aa phosphoprotein. It is rich in Proline and Arginine residues and contains a C-terminal Leu-zipper (aa286-313) that is known to interact with the metal-binding motif of ATF-2. Unlike mouse UTF1 which contains an alternate start site at Met 43, human UTF1 lacks this Methionine and shows only one isoform. Over aa44-153, human UTF1 shares 81% aa sequence identity with mouse UTF1.\n\nApplications:\nWestern Blot: 1:500-1:2000\nFlow Cytometry: 1:200-1:400\nELISA: 1:10000\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

030714

Size

100ug

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

Form

Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1.Int J Exp Pathol. 2011 Oct;92(5):326-32.\n 2.Stem Cell Res. 2009 May;2(3):211-8.

Additional Info

Human

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