V5 Epitope Tag GKPIPNPLLGLDST (Pk-tag, Paramyxovirus, SV5) (Biotin)

Supplier:

United States Biological

Cat no: V0500-00X

V5 Epitope Tag GKPIPNPLLGLDST (Pk-tag, Paramyxovirus, SV5) (Biotin)

Recommended Secondary Reagents: Streptavidin: HRP\n\nFusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.\n\nApplications:\nWestern Blotting: 1:1000-1:5000\nELISA\nImmunohistochemistry (frozen)\nOptimal working dilutions to be determined by researcher.

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SPECIFICATIONS

Catalog Number

V0500-00X

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Mouse

Conjugates

Biotin

Form

Purified IgG conjugated to Biotin. Supplied as a liquid with 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Southern, J.A., Young, D.F., Heaney, F., Baumgartner, W. & Randall, R.E. (1991). Identifcation of an epitope on the P & V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics. Journal of Gener. Acknowledgement: This product is manufactued under an exclusive license from the University of St. Andrews, UK.

Additional Info

Recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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