Vaccinia Virus B5R (Smallpox, VACV, VV)

B5R, a 561aa membrane protein containing ankyrin repeats that mediate protein-protein interactions and is related to the regulators of complement activation (RCA) superfamily. It consists of four short consensus repeats (SCRs) and transmembrane anchor region or the CT (cytoplasmic tail). Its transmembrane domain is the major determinant for targeting the B5R protein to the outer membrane of EEV (extracellular enveloped virus) and for supporting EEV formation, thus playing an important role in the process of virus envelopment and release. It may also be involved in viral evasion from host immune responses. B5R is essential for efficient wrapping of IMV, actin tail formation, normal plaque size, virus virulence, incorporation of the protein into EEV and for EEV formation.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nA partial recombinant fusion protein\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1014-58V4

Size

100ug

Applications

WB

Hosts

Mouse

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography

Isotype

IgG1,k

References

1. Massung,R.F., Loparev,V.N., Knight,J.C., Totmenin,A.V., Chizhikov,V.E., Parsons,J.M., Safronov,P.F., Gutorov,V.V., Shchelkunov,S.N. and Esposito,J.J. Terminal region sequence variations in variola virus DNA. Virology 221(2), 291-300 (1996).

Additional Info

Recognizes variola virus Smallpox B5R. \nSpecies Sequence Homology: cowpox virus, camelpox, taderapox, 93%; vaccinia virus, 86%; ectromelia virus, rabbitpox virus and monkeypox virus, 80%