Vaccinia Virus (Smallpox, VACV, VV, Lister strain) \n(Azide Free)

Vaccinia virus is closely related to the virus that causes cowpox. The precise origin of vaccinia virus is unknown due to the lack of record-tracking as the virus was repeatedly cultivated and passaged in research laboratories for many decades. The most common notion is that vaccinia virus, cowpox virus and variola virus (the causative agent for smallpox) were all derived from a common ancestral virus. There is also speculation that vaccinia virus was originally isolated from horses.\n\nApplications: \nSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): Use Proteinase K digestion for antigen retrieval.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

V0500-10A

Size

1ml

Applications

ELISA, IHC

Hosts

Rabbit

Form

Supplied as a liquid in PBS, pH 7.2. No stabilizing proteins added. No preservative added. Also available with sodium azide. See V0500-10.

P Type

Pab

Purity

Purified by Protein A affinity chromatography ((same/more than) 95%).

Isotype

IgG

Additional Info

Recognized purified virions. Crossreactive with Lister, Wyeth, New York City and MVA strains of Vaccinia. Does not crossreact with parainfluenza (1-3), RSV, adenovirus, influenza A & B or HSV-1. Does not crossreact with uninfected cells.