VAMP 1,2 (Vesicle-Associated Membrane Protein, Synaptobrevin)

Vesicle-associated membrane protein (VAMP), also known as synaptobrevin, is an 18kD integral membrane protein localized to the cytoplasmic surface of synaptic vesicle (2). VAMP consists of a proline-rich amino-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a carboxyl-terminal tail (3). Two VAMP homologs, VAMP1 and VAMP2, are differentially expressed in the nervous system (3). VAMP1 expression is localized to neurons involved in modulating overlapping patterns, whereas VAMP2 is found in neurons associated with autonomic, sensory and integrative functions. In non-neuronal tissues, VAMP1 is restricted to pancreas and kidney tubular cells, whereas VAMP2 is predominantly expressed in Langerhans islets and glomerular cells (4). Evidences for VAMP

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

V2024-01A

Size

25ul

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Prc, Xen/Amph

Form

Supplied as a liquid in PBS, pH 7.2, 0.1mM PMSF, 50% glycerol.

P Type

Mab

Purity

Purified by ammonium sulfate pecipitation.

Isotype

IgM

References

1. Honer, W.G., et al., Brain Rsch. 609: 9-20 (1993). 2. Calakos, N. & Scheller, R., Physiological Reviews 76: 1-29 (1996). 3. Trimble, W.S., et al., J. Neurosci. 10: 1380-1387 (1990). 4. Rossetto, O., et al., J. Cell Biology 132: 167-179 (1996). 5. Protopopov, V., et al., Cell 74: 855-861 (1993). 6. Schiavo, G., et al., Nature 359: 832-835 (1992). 7. Sollner, T., et al., Cell 75: 409-418 (1993). 8. Ferrer-Montiel, A.V., et al., FEBS Lett. 435: 84-88 (1998). 9. Cho, S.J., et al., Proc. Natl. Acad. Sci. USA 99(7): 4720-4724 (2002).

Additional Info

Recognizes human VAMP on SDS-PAGE immunoblots at ~18kD. Recognizes the VAMP1 aa1-118 and the VAMP2 aa33-96-GST fusion proteins expressed in E. coli. No reactivity was seen with a ~18kD protein in mouse liver extracts. Species Crossreactivity: mouse, rat, bovine, hamster, porcine and Xenopus.