VAP-1 (Vascular Adhesion Protein 1, Amine Oxidase Copper-Containing 3, AOC3, Semicarbazide-Sensitive Amine Oxidase, SSAO, HPAO, Membrane copper amine oxidase, Semicarbazide-sensitive amine oxidase)

Vascular Adhesion Protein 1 (VAP-1), also called AOC3 (amine oxidase copper-containing 3) or SSAO (semicarbazide-sensitive amine oxidase) is a type II transmembrane sialoglycoprotein that is upregulated on endothelial cells by inflammation. The 170kD disulfide-linked homodimer of 763 aa units is an ectoenzyme that deaminates amines, forming aldehydes and hydrogen peroxide. It also mediates lymphocyte rolling, adhesion and possibly extravasation during inflammation. Adipocytes can release a soluble form via metalloproteinase cleavage. Human and mouse VAP-1 share 83% aa identity.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:64000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

V2095-02E

Size

100ug

Applications

ELISA

Hosts

Goat

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Forster-Horvath C, Dome B, Paku S, Ladanyi A, Somlai B, Jalkanen S, Timar J. Loss of vascular adhesion protein-1 expression in intratumoral microvessels of human skin melanoma. Melanoma Res. 2004 Apr;14(2):135-40.

Additional Info

Recognizes human AOC3. Species Crossreactivity: Mouse and rat.