Vascular Endothelial Growth Factor C, Propeptide (VEGF-C, VEGF-A, Vascular permeability factor, VPF)

Vascular endothelial growth factors (VEGF) are structurally and functionally related growth factors, that may play important roles in the formation of vascular systems during embryonic development, in regulation of capillary growth in adults, and in maintenance of normal vasculature. VEGF-C is a ligand for receptor tyrosine kinase VEGFR-3 (Flt-4), which is mainly expressed in the endothelium of lymphatic vessels. VEGF-C mRNA has been detected in human heart, lung, muscle, ovary, placenta, and small intestine. It is also detected in many malignant tumors, including breast carcinomas, squamous cell carcinomas, lymphomas, melanomas, sarcomas and ademomas. VEGF-C is synthesized as a precursor protein called prepropeptide, which undergoes a series of proteolytic steps to give the mature form of VEGFC. The prepropeptide is cleaved and forms a homo-dimer between two identical propeptides. This dimer is then processed to form a secretable tetramer that consists of two 29kD and two 31kD peptides, which is then secreted extracellularly and processed to produce the mature form that consists of two 21kD peptides. Studies suggest the 29/31kD form is the most prevalent form of VEGF-C in many biological systems.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications have not been tested.\n\nRecommended Dilutions:\nELISA (Immunogen): 0.1-1ug/ml\nWestern Blot (Human): 2-5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nU937 lymphoma cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

V2121-30A

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Li X et al. Proc Natl Acad Sci. 95(24): 14389-94 (2001). 2. Salven P et al. Am J Pathol 153(1): 103-8 (1998). 3. Partanen TA et al. Faseb J. 14(13): 2087-96 (2000). 4. Valtola R et al. Am J Pathol. 154(5): 1381-90 (1999). 5. Olofsson J et al. Cell Struct. Funct. 21:381-385 (1999). 6. Joukov V et al. Embo J. 16(13): 3898-911 (1997).

Additional Info

Recognizes human VEGF-C propeptide. Does not recognize the mature form of VEGF-C.