Vascular Endothelial Growth Factor Receptor 2, phosphorylated (Tyr1054, Tyr1059) (VEGF R2, Flk1, KDR, Receptor Tyrosine Kinase)

Vascular Endothelial Growth Factor Receptor 2 (VEGFR2, also known as KDR/FLK-1) is a ~200kD member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors upon ligand binding, VEGFR2 forms a dimer and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2- and phosphotyrosine binding domain containing signaling proteins. Phosphorylation of tyrosines 1054 and 1059 in the activation loop is required for activation of VEGFR2 and its intrinsic tyrosine kinase activity.\n\nApplications:\nSuitable for use in Western blot. Other applications not tested.\n\nRecommended Dilution: \nWestern Blot: 1:1000\nOptimal dilution to be determined by researcher. \n\nPositive Controls:\nPorcine aortic endothelial (PAE) cells transfected with a chimeric receptor consisting of the extracellular portion of the CSF-1 receptor fused with the transmembrane and intracellular domains of mouse VEGFR2.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

V2110-19D

Size

10 Blots

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in Dulbecco PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG and Protease free), 50% glycerol , pH 7.3, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography

Isotype

IgG

References

1. Meyer, R.D., et al. (2002) The presence of a single tyrosine residue at the carboxyl domain of vascular endothelial growth factor receptor-2/FLK-1 regulates its autophosphorylation and activation of signaling molecules. J. Biol. Chem. 277(30):27081-27087. 2. Zeng, H., et al. (2001) Tyrosine residues 951 and 1059 of vascular endothelial growth factor receptor-2 (KDR) are essential for vascular permeability factor/vascular endothelial growth factor-induced endothelium migration and proliferation, respectively. J. Biol. Chem. 276(35):32714-32719. 3. Sato, Y., et al. (2000) Properties of two VEGF receptors, Flt-1 and KDR, in signal transduction. Ann. N.Y. Acad. Sci. 902:201-207. 4. Rahimi, N. and A. Kazlauskas (1999) A role for cadherin-5 in regulation of vascular endothelial growth factor receptor 2 activity in endothelial cells. Mol. Biol. Cell 10(10):3401-3407.

Additional Info

Recognizes human VEGFR2 when phosphorylated at Tyr1054 and Tyr1059. Sequence Homology (100%): VEGFR3. Species Crossreactivity: Mouse.