Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF)

VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF189 and VEGF206) remain cell associated.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry . Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nImmunohistochemistry (Paraffin): 1:50\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: VEGF 165 recombinant protein.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

V2110-08D

Size

500ul

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

11. Brown LF et al., Journal of Investigative Dermatology, (1995) 104:744-749. 2. Brown LF et al., J of Immunology, (1995) 154:2801-2807. 3. Claffey KP et al., Biochimica et Biophysica Acta, (1995) 5, 1246:1-9. 4. Dvorak HF et al., American Journal of Pathology, (1995) 146:1029-39. 5. Fischer S et al., Brain Research. Molecular Brain Research, (1995) 28:141-8. 6. Frank S et al., J. Biol. Chem. (1995) 270:12607-12613. 7. Hatva E. et al., American Journal of Pathology, (1995) 146:368-78. 8. Kamat BR et al., American Journal of Pathology, (1995) 146: 157-165. 9. O'Brien T et al., Cancer, (1995), 75:2867-2874. 10. Pierce EA et a., PNAS (1995) 92:905-909. 11. Qu-Hong et al., Journal of Histochemistry and Cytochemistry, (1995) 43:381-389. 12. Samoto K et al., Cancer Research (1995) 55:1189-1193. 13. Sharma HS et al., Biochimica et Biophysica Acta (1995)1260:235-238. 14. Shweiki D et al., PNAS (1995) 92:768-772. 15. Simon M et al., American Journal of Physiology (1995) 268:F240-F250. 16. Stavri GT et al., Febs Letts. (1995) 358:311-315. 17. Takahashi T et a., BBRC (1995) 209 :218-226. 18. Tuder RM et al., Journal of Clinical Investigation (1995) 95:1798-1807. 19. Aiello LP et al., New England Journal of Medicine, (1994) 331:1480-1487. 20. Banai S et al., Cardiovascular Research (1994) 28:1176-1179. 21. Fava RA et al., Journal of Experimental Medicine (1994) 180:341-346. 22. Boocock CA; et al. Journal of the National Cancer Institute (1995), 87:506-516. 23. Brown LF; et al. Human Pathology (1995) 26:86-91.

Additional Info

Recognizes human VEGF at ~19-22kD. Species Crossreactivity: mouse.