Vascular Endothelial Statin (VE-Statin, EGF-Like Domain-containing Protein 7, EGFL7)

Mouse VE-Statin (vascular endothelial cell statin; also EGFL7) is a 41kD, secreted glycoprotein that belongs to an expanding family of EGF-like domain-containing proteins. It is an early marker of embryonic endothelial cells, and occurs in adult endothelium. Its secretion blocks smooth muscle migration. The mature molecule is 254 amino acids (aa) in length, and contains one N-terminal EMI domain (aa28-105), two EGF-like domains (aa 106-178), and a coiled-coil region (aa196-220). There is at least one isoform that shows a 13 aa deletion between aa236-248. Mature mouse VE-statin is 76% and 80% identical to dog and human VE-statin, respectively. \n\nApplications:\nSuitable for use in Western Blot and Direct ELISA. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-2ug/ml. The detection limit fot recombinant mouse VE-Statin is ~25ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity 5 to 50 fold.\nDirect ELISA: 0.5-1ug/ml. The detection limit fot recombinant mouse VE-Statin is ~7ng/well.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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