Vasoactive Intestinal Peptide (VIP)

VIP is localized in nerve fibers of the central and peripheral nervous system, and is probably acting as a neurotransmitter. Smooth muscle relaxation, vasodilation and secretion from exocrine glands are some of the effects of VIP. The Verner-Morrison or Watery Diarrhea Hypokaliemia and Achlorhydria (WDHA) syndrome is a characteristic clinical syndrome associated with overproduction of VIP from endocrine tumors. These VIP-producing tumors are usually neuroblastomas of endocrine tumors in the pancreas. \n\nApplications: \nSuitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunofluorescence: 1:1000-1:2000 with overnight incubation at 4 degrees C\nImmunohistochemistry: Frozen and paraffin sections\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nStefanini-fixed frozen sections of rat intestine.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

V2115-06

Size

50ul

Applications

IF, IHC

Reactivities

Hum, Rat, Fel, GP, Prc, Rabbit

Form

Supplied as a lyophilized powder. Reconstitute with 50ul sterile ddH2O. Further dilutions can be made in PBS, 1% BSA, 0.1% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Messel T et al. Extrinsic control of the release of galanin and VIP from intrinsic nerves of isolated, perfused porcine ileum. Reg Pept 38, 179-198 (1992). 2. Luts A et al. Peptide-containing nerve fibers in human airways: Distribution and coexistence pattern. Arch. Allergy Immunol. 101, 52-60 (1993). 3. Ny L et al : Nitric oxide synthase-containing, peptide-containing and acetylcholinesterase-positive nerves in the cat lower oesophagus. Histochem J 26, 721-733 (1994). 4. Lopez-Figueroa MO et al. Presence of nitric oxide synthase in the sheep pineal gland: An experimental immunohistochemical study . Neuroendocrinology 63, 384-392 (1996).

Additional Info

Recognizes human VIP. Absorption with 10-100ug immunogen/ml diluted antiserum abolishes the staining, while PHI does not. Species Crossreactivity: porcine, feline, rabbit, rat, guinea pig.