VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF)

VEGF which is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF189 and VEGF206) remain cell associated.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested. \n\nRecommended Dilution: \nWestern Blot: 1-2ug/ml for 2 hours at RT\nImmunohistochemistry (formalin/paraffin):1:50-1:100 for 30 min at RT. Staining of formalin-fixed tissues requires boiling\ntissue sections in 1mM EDTA, pH 8.0, for 10-20 min. followed by cooling at RT for 20min.\nOptimal dilution determined by the researcher. \n\nPositive Control: \nVEGF165 recombinant protein., Angiosarcoma.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

V2110-16B

Size

250ul

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide before the addition of glycerol to 40%. Also available without BSA and azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Turley, H., et al., J. Pathol. 186: 313-318 (1998). 2. O

Additional Info

Recognizes human 121, 165 and 189 isoforms of VEGF1.