Vesicular GABA Transporter (solute carrier family 32, member 1, bA122O1.1, GABA and Glycine transporter, hVIAAT, Solute carrier family 32 member 1, Vesicular GABA transporter, Vesicular inhibitory amino acid transporter, VGAT, VIAAT)

Vesicular GABA transporter (VGAT), the first vesicular amino acid transporter to be molecularly identified, differs in structure from previously cloned vesicular neurotransmitter transporters and defines a novel gene family. VGAT is highly concentrated in the nerve endings of GABAergic neurons in the brain and spinal cord but also in glycinergic nerve endings. In contrast, hippocampal mossy fiber boutons, which are glutamatergic but known to contain GABA, lack VGAT immunoreactivity. Although the great majority of nerve terminals containing GABA or glycine are immunopositive for VGAT, subpopulations of nerve endings rich in GABA or glycine appear to lack the protein. Additional vesicular transporters or alternative modes of release may therefore contribute to the inhibitory neurotransmission mediated by these two amino acids.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:500-1:1000 \nImmunohistochemistry: 1:50-1:500\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nRat brain lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

V2121-87B

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 15mM sodium chloride, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Tabuchi, Katsuhiko, et al.. (2007) Science, 318: 71-6. 2. Chessler, SD et al.. (2002) Diabetes, 51:1763-1771 3. McIntire, S.L. et al.. (1997) Nature, 389:870-876.

Additional Info

Recognizes rat VGAT at ~57kD. Species Sequence Homology: human, mouse and monkey.