Vimentin (FLJ36605, VIM)

Vimentin is an intermediate filament with a molecular weight of ~58kD, and an average diameter of 10 nm. Within the same species, the central domain of the filament is 30% homologous to cytokeratin, desmin, neurofilaments, and glial fibrillary acidic protein. Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. It is usually co-expressed with cytokeratin in some epithelial cells such as Sertoli cells. Vimentin expression may change as a tumor becomes more aggressive, as in the case of hyperplastic prostate where prostatic intraepithelial neoplasia strongly expresses vimentin (91%), but invasive carcinoma is minimally reactive with Mouse anti-Vimentin. Vimentin is useful as part of an antibody panel for differential diagnostic purposes. It may be included in an antibody panel for differentiating soft tissue tumors. For example, co-expression of vimentin and cytokeratin is indicative of epitheloid sarcoma. In one case, a differential antibody panel using vimentin, cytokeratin, CEA, and EMA distinguished reactive mesothelial cells from malignant epithelial cells in the cytodiagnosis of serous effusions in 39/46 cases (85%).\n\nApplications:\nSuitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nImmunohistochemistry: Formalin-fixed, paraffin-embedded (FFPE) tissue sections.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nEndometrial carcinoma or malignant fibrous histiocytoma.\n\nExpected Staining Pattern: Predominantly cytoplasmic\nEpitope Retrieval: Not Required\nEnzyme Digestion: Not Required\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

V2122-02A

Size

1ml

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Rat, Ch/Bird

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.

P Type

Mab

Purity

Purified from mouse antisera

Isotype

IgG1,k

References

1. Nagle RB, et al. Am J Pathology 138(1): 119-128, 1991. 2. Daste G, et al. Cytopathology 2(1): 19-28, 1991. 3. Osborn M, et al. Eur J Cell Biol 34:137-143, 1984.

Additional Info

Specific for vimentin and does not recognize any other intermediate filaments, including desmin and glial fibrillary acidic protein. Reacts with human vimentin expressed by Hela, K562, and Wi38 cells, and recognizes a ~58 kD protein on Western blot analysis. Species Crossreactivity: rat and chicken. Does not recognize vimentin found in mouse 3T3 cells.