Vimentin (FLJ36605, VIM)

Vimentin is an intermediate filament with a molecular weight of ~58kD, and an average diameter of 10 nm. Within the same species, the central domain of the filament is 30% homologous to cytokeratin, desmin, neurofilaments, and glial fibrillary acidic protein. Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. It is usually co-expressed with cytokeratin in some epithelial cells such as Sertoli cells. Vimentin expression may change as a tumor becomes more aggressive, as in the case of hyperplastic prostate where prostatic intraepithelial neoplasia strongly expresses vimentin (91%), but invasive carcinoma is minimally reactive with Mouse anti-Vimentin. Vimentin is useful as part of an antibody panel for differential diagnostic purposes. It may be included in an antibody panel for differentiating soft tissue tumors. For example, co-expression of vimentin and cytokeratin is indicative of epitheloid sarcoma. In one case, a differential antibody panel using vimentin, cytokeratin, CEA, and EMA distinguished reactive mesothelial cells from malignant epithelial cells in the cytodiagnosis of serous effusions in 39/46 cases (85%).\n\nApplications: \nSuitable for use in Immunohistochemistry, Immunofluorescence, ELISA and Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nImmunohistochemistry (Frozen and Paraffin): 1:2000. Minimum of 1 hour incubation. Longer for paraffn sections. Protease pretreatment is recommended for paraffin sections.\nWestern Blot: Band at ~57kD\nOptimal dilutions to be determined by the researcher.\n\nSource: \nAscites fluid\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

V2122-03

Size

50ug

Applications

ELISA, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Bov, Can, Ch/Bird, NHP

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

Franke, W. W. & Moll, R. Differentiation 36, 145

Additional Info

Recognizes cells of mesenchymal derivation, including endothelial cells and certain smooth muscle cells of the vascular tract, fibroblasts, connective tissue, all types of blood cells, including thymocytes, interstitial and testicular Sertoli cells, ovarian follicle cells. Can also be used to detect co-expression of vimentin in combination with other intermediate filament proteins. Species Crossreactivity: bovine, canine, chicken, human, monkey and amphibian..