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Vimentin (VIM)

Vimentin (VIM)

Cat no: V2122-08B

Supplier: United States Biological
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Vimentin is a protein belonging to the class of intermediate filaments of the cell, which includes other proteins such as keratins, desmin, neurofilaments, and nuclear lamins. It is present in the majority of cells of mesenchymal and nonmesenchymal origin, and its filaments are associated with both the nuclear and plasma membranes. It maps to 10p13 region of human chromosome. It is highly expressed in fibroblasts and is also expressed in many hormone-independent mammary carcinoma cell lines. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry (formalin fixed paraffin embedded): 1:25-1:50 Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher. Positive Control: Sarcoma Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: V2122-08B
Reactivities: Human, Rat, Canine, Chicken/Bird, Feline, Porcine
Hosts: Mouse
Applications: Immunohistochemistry, Western Blot
Size: 500ul
Form: Supplied as a liquid, 0.05% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified
References: 1. Osborn et al. J Cell Biol 34: 137, 1984. 2. Azumi et al. Am J Pathol 88: 286, 1987 3. Buley et al. J Clin Pathol 40: 136, 1987. 4. Styers et al. J Cell Sci 119: 3643, 2006. 5. Ferrari et al. Mol Cell Biol 6: 3614, 1986.
Additional info: Recognizes porcine Vimentin. Species Crossreactivity: feline, chicken, canine, equine, human, rat.

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