Von Willebrand Factor (vWF) BioAssay(TM) ELISA Kit (Antibodies only)

Matched Matched-Pair Antibody Set suitable for use in sandwich ELISA for the detection of human von Willebrand Factor Antigen (vWF). \n\nvon Willebrand Factor (vWF, previously referred to as Factor VIII Related Antigen) is a large adhesive protein produced in endothelial cells and megakaryocytes. There are two critical functions of vWF. The first being its involvement in the process of platelet adhesion and aggregation through interaction with platelet receptor glycoprotein Ib. The second being the binding and stabilization of Factor VIII (anti-hemophilic factor) for secretion and transport in plasma. The vWF precursor protein is synthesized with a 95kD propeptide (also known as vWF antigen-II). The propeptide is believed to be involved in the intracellular multimerization of the vWF subunits. The mature vWF multimers are then packed into storage organelles within the cell (Weibel-Palade bodies). After which, the propeptide is cleaved and released. vWF circulates as multimers of disulfide linked 220kD subunits and the molecular weight of these multimers ranges from 0.5-20 million daltons. The plasma concentration of vWF is typically 10ug/ml. Increased levels are often observed in pregnancy and other conditions of physiological stress. von Willebrand

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SPECIFICATIONS

Catalog Number

V2700-09

Size

1Kit

Applications

ELISA

Form

Supplied as a liquid in 50% glycerol.

References

1. Montgomery, R.R., et al., in Hemostasis and Thrombosis, 3rd Edition, eds. R.W. Colman, et al., pp. 134-168, J.B. Lippincott Co., Philadelphia PA, USA (1994). 2. Sadler, J.E., JBC 266(34): 22,777-22,780 (1991). 3. Bartlett, A., et al., Brit. Med. J. 1: 994-996 (1976). 4. Nix, B. & Wild D., in Immunoassays, A Practical Approach, editor J.P. Gosling, pp. 239-261, Oxford University Press (2000). 5. Evaluation of the Linearity of Quantitative Analytical Methods; Proposed Guidline: Second Edition. NCCLS Document EP6-P2 (ISBN 1-56238-446-5, NCCLS, Wayne, Pennsylvania USA, (2001). 6. FDA Guidance for Industry. Bioanalytical Method Validation; May (2001) (www.fda.gov/cder/guidance/index.htm)